Catalog #SIM0085
InVivoSIM anti-human IL-6R (Sarilumab Biosimilar)
Clone
Sarilumab
Reactivities
Human, Monkey
Applications
In vivo functional assays
in vitro functional assays
Flow cytometry
Western blot
ELISA
in vitro functional assays
Flow cytometry
Western blot
ELISA
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Sarilumab, making it ideal for research use. Sarilumab is a humanized IgG1 monoclonal antibody that reacts with the interleukin 6 receptor (IL-6R), also known as CD126, IL6RA, gp80, IL6Q, and HIES5. IL-6R is an 80 kDa potent pleiotropic type I cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL-6R is expressed by activated T and B lymphocytes, monocytes, hepatocytes, plasma cells, and retinal endothelial cells. The IL-6R α chain binds IL-6 but requires complexing with gp130 to initiate signaling pathways (e.g., the Jak/STAT and the MAPK pathways). The IL-6R complex is involved in antigen-specific immune responses, inflammatory responses, and neuronal development and is a major mediator of the acute phase reaction. Monoclonal antibodies, such as Sarilumab, are useful for blocking IL-6R activation in in vitro and in vivo research experiments by binding to and neutralizing the function of IL-6R. Sarilumab is reported to bind monomeric IL-6R and dimeric IL-6R Fc-fusion protein with high affinity (over 15x higher than tocilizumab). Sarilumab is reactive against human and cynomolgus monkey species, but it does not bind mouse IL-6R.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human IL-6R |
| Reported Applications |
In vivo functional assays in vitro functional assays Flow cytometry Western blot ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |