Your hybridoma or cell line.
Purified antibody back to you in 4 to 6 weeks.
Endotoxin-controlled contract antibody manufacturing for established preclinical programs. Milligram to multi-gram lots. U.S.-based production. Identical formulation and QC standards from pilot through program scale. Hybridoma scale-up, stable cell line production, and recombinant source manufacturing available.
Antibody Manufacturing Designed for Mid-Scale Programs
Bio X Cell was built for the scale between discovery and clinical: 25 mg to multi-gram lots, endotoxin-controlled, in vivo-ready, with an average 4 to 6 week turnaround. The platform behind ~30,000 peer-reviewed citations, available for your custom program.
Production workflow and antibody delivery
Define requirements and confirm specifications
Provide starting material details, target lot size, formulation requirements, and study timeline. Bio X Cell confirms the production plan and endotoxin specifications at project scoping. No ambiguity at lot release.
Transfer starting material
Ship hybridoma cells, stable cell line, or defined production materials. Bio X Cell immediately initiates production. Communication for a dedicated project manager throughout. Optional cell banking keeps the line secure for seamless resupply.
Receive purified antibody
Protein A or G purification, buffer exchange to PBS (no preservatives, no stabilizers), sterile filtration. Antibody formulated to client specifications, custom-vialed, and shipped with a per-lot Certificate of Analysis. Average 4 to 6 weeks from receipt of starting material.
Hybridoma Scale-Up and Cell Line Services
Bio X Cell manages your hybridoma or stable cell line from transfer through purified antibody delivery, including support preparing and shipping your cells to the facility.
Cell banking and storage
Scale-up and purification
Isotype confirmation
Mycoplasma & pathogen testing
Antibody Production Capabilities for Preclinical Research
| Phase | Description | Included | Optional Add-Ons |
|---|---|---|---|
| Pilot | Instructions for preparing and shipping cells |  |  |
| Small-scale assessment of cell line activity with Protein A or G purification | | |
|
| Mycoplasma testing | | |
|
| Antibody isotyping | | |
|
| Cell Culture | Cell culturing and monitoring (7 days/week) | | |
| Expansion of cells using optimized culture processes | | |
|
| Freezing of five vials of cells (supports future production continuity) | | |
|
| Storage of cells at Bio X Cell (enables rapid re-supply) | | |
|
| Purification | Purification of supernatant using Protein A or G | | |
| Buffer exchange to PBS (preservative- and stabilizer-free) | | |
|
| Sterile filtration of final product | | |
|
| Endotoxin assessment (&le1 EU/mg standard; further purification available) | | |
|
| Vialing & QC | Sterile vialing of finished product | | |
| Custom aliquoting | | |
|
| Internal antibody quality assessment | | |
|
| Certificate of Analysis (CoA) | | |
|
| Murine pathogen screening | | |
|
| Aggregation analysis by SEC (aggregate profile characterization) | | |
|
| Shipping | Cold-pack shipping in environmentally friendly packaging | | |
Contract Antibody Production Services: Frequently Asked Questions
Purified, characterized antibody lots. A per-lot Certificate of Analysis documenting all QC results ships with the antibody.
Yes. Provide your hybridoma cells and Bio X Cell handles cell banking, culture scale-up, Protein A or G purification, QC testing, and delivery of purified antibody lots. Pilot production is available before committing to full-scale runs. Cell banking secures the line for ongoing supply.
Bio X Cell routinely achieves endotoxin levels as low as ≤0.1 EU/mg. Endotoxin is controlled during manufacturing, not corrected after purification. Program-defined specifications for sensitive model systems are set at project scoping and tested on every lot before release.
Bio X Cell supports production from 25 mg up to multi-gram quantities. Lot size is defined by program requirements and confirmed during project scoping.
Large CDMOs are optimized for clinical-scale GMP manufacturing and are not designed for flexible research-use supply. Bio X Cell serves the mid-scale layer: 25 mg to multi-gram lots, research-use cost profile, endotoxin-controlled manufacturing, and 4 to 6 week average turnaround. Over 1,000 custom projects completed.
Contact Bio X Cell to confirm starting material, target lot size, and study specifications. A Bio X Cell scientist works with you from the start, guiding cell preparation and shipment through to target specifications and QC. Projects begin within days of starting material receipt, with an average 4 to 6 week turnaround.
No. Bio X Cell does not provide antibody discovery, immunization, or hybridoma generation. Bio X Cell engineers and produces antibodies from an existing starting point: your sequence, construct, hybridoma, or a Bio X Cell catalog clone.
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Whether you need antibody customization or high-volume production, Bio X Cell is committed to advancing your therapeutic innovations.
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